Synchrotron Radiation X-ray Tomographic Microscopy (SRXTM) is a powerful non-destructive imaging technique that utilizes the highly intense and tunable X-ray beam generated by a synchrotron light source. This X-ray beam is directed towards the sample of interest. The differential absorption of X-rays by the various components within the sample creates a characteristic transmission pattern.

           To capture this pattern, a scintillator converts the transmitted X-rays into visible light. This light is then magnified by an objective lens system coupled to a high-resolution camera, resulting in a detailed X-ray image. For a complete 3D reconstruction, SRXTM acquires multiple X-ray images by rotating the sample through a specified angular range, typically at least 180 degrees. If the sample size exceeds the field of view of the detector, multiple scans may be necessary.

           Following image acquisition, a series of calibration steps are performed. Flat-field correction, using both bright and dark current images, removes artifacts caused by camera sensitivity variations and optical path distortions. Preprocessing often includes noise reduction and beam intensity normalization to further enhance image quality. The preprocessed images serve as input for computational reconstruction. Employing the filtered back-projection algorithm, these images are transformed into sinograms, which are then used to generate the final 3D tomographic images. This collection of reconstructed slices, also referred to as tomograms, provides a comprehensive visualization of the sample's internal structure with high resolution in all three dimensions.