Synchrotron radiation X-ray Tomographic Microscopy (SRXTM) utilizes the x-ray beam generated from the synchrotron light source in the x-ray imaging.  When the x-ray beam is projected onto the sample, the x-ray beam that passes through the sample (transmitted x-ray beam) creates an x-ray pattern from the differential absorption of the sample. To acquire the x-ray image, the scintillator is used to convert the transmitted x-ray to visible light.  Then it is magnified by the objective-lens coupled microscope and finally recorded by the high-resolution camera.  A typically SRXTM scan is carried out by acquiring the x-ray images around the sample for at least 180 degrees (if the sample is larger than the available field of view of the detection system, multiple scans will be required to satisfy tomographic mathematical calculation).  After the scan, all x-ray images are calibrated by flat-field correction with bright and dark current images to remove the effects of image artifacts caused by variations in the pixel-to-pixel sensitivity of the camera and by distortions in the optical path. The preprocessing usually includes noise filtering and beam intensity normalization. The resulting x-ray images are inputted in computational processing to obtain the sinograms and the reconstructed tomographic images based on the filtered back-projection algorithm. The collection of reconstructed images (also referred to as "tomograms") reflects the cross-sectional details of the sample in three dimensions.